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anti folr1  (Proteintech)


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    Structured Review

    Proteintech anti folr1
    Anti Folr1, supplied by Proteintech, used in various techniques. Bioz Stars score: 93/100, based on 26 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/folr1+antibody/FOLR1+Antibody/pm41047036-93-13-14
    Average 93 stars, based on 26 article reviews
    anti folr1 - by Bioz Stars, 2026-09
    93/100 stars

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    Related Articles

    Western Blot:

    Article Title: Novel Anti-FOLR1 Antibody–Drug Conjugate MORAb-202 in Breast Cancer and Non-Small Cell Lung Cancer Cells
    Article Snippet: Equal amounts of protein samples were separated by 10% SDS-PAGE electrophoresis and transferred onto polyvinylidene fluoride (PVDF) membranes (Merck Millipore; Burlington, MA, USA). .. Membranes were blocked with 5% skim milk for 1 h. Immunoblotting was performed by incubating the membranes overnight with FOLR1 antibody (60307-1-1; Proteintech; Rosemont, IL, USA), and β-actin antibody (4970; Cell Signaling Technologies; Danvers, MA, USA) as primary antibodies (diluted with Tris Buffered Saline with Tween 20 (TBST) according to manufacturer’s instructions, 1:1000 to 1:2000). .. After washing with TBS-0.05% Tween, membranes were incubated with anti-mouse immunoglobulin (Ig)G, HRP-linked antibody (7076; Cell Signaling Technologies Danvers, MA, USA) or anti-rabbit IgG, and horseradish peroxidase (HRP)-linked antibody (7074; Cell signaling Technologies) as secondary antibodies (diluted with TBST according to manufacturer’s instructions, 1:1000 to 1:2000).

    Saline:

    Article Title: Novel Anti-FOLR1 Antibody–Drug Conjugate MORAb-202 in Breast Cancer and Non-Small Cell Lung Cancer Cells
    Article Snippet: Equal amounts of protein samples were separated by 10% SDS-PAGE electrophoresis and transferred onto polyvinylidene fluoride (PVDF) membranes (Merck Millipore; Burlington, MA, USA). .. Membranes were blocked with 5% skim milk for 1 h. Immunoblotting was performed by incubating the membranes overnight with FOLR1 antibody (60307-1-1; Proteintech; Rosemont, IL, USA), and β-actin antibody (4970; Cell Signaling Technologies; Danvers, MA, USA) as primary antibodies (diluted with Tris Buffered Saline with Tween 20 (TBST) according to manufacturer’s instructions, 1:1000 to 1:2000). .. After washing with TBS-0.05% Tween, membranes were incubated with anti-mouse immunoglobulin (Ig)G, HRP-linked antibody (7076; Cell Signaling Technologies Danvers, MA, USA) or anti-rabbit IgG, and horseradish peroxidase (HRP)-linked antibody (7074; Cell signaling Technologies) as secondary antibodies (diluted with TBST according to manufacturer’s instructions, 1:1000 to 1:2000).

    Article Title: KDM6B Variants May Contribute to the Pathophysiology of Human Cerebral Folate Deficiency.
    Article Snippet: .. 48 h after transfection, cells were fixed with 4% paraformaldehyde for 30 min and cell membranns were permeabilized with 0.3% Triton X-100 in TBST for 20 min, and then blocked with 10% normal goat serum (NGS) in Tris-buffered saline with 0.1% Tween® 20 Detergent (TBST) for 1 h. Cells were then incubated with FOLR1 antibody (1:100, Proteintech, Rosemont, IL, USA, 23355-1-AP) and flag antibody (1:1000, ThermoFisher Scientific, MA1-91878) overnight at 4 ◦C. .. After incubation, cells were rinsed with Phosphate-buffered saline (PBS) and incubated with secondary antibodies (1:1000, Cell Signaling Technology, Danvers, MA, USA, 8889S; 4408S) for 1 h, avoiding light exposure.

    Article Title: KDM6B Variants May Contribute to the Pathophysiology of Human Cerebral Folate Deficiency
    Article Snippet: .. 48 h after transfection, cells were fixed with 4% paraformaldehyde for 30 min and cell membranns were permeabilized with 0.3% Triton X-100 in TBST for 20 min, and then blocked with 10% normal goat serum (NGS) in Tris-buffered saline with 0.1% Tween ® 20 Detergent (TBST) for 1 h. Cells were then incubated with FOLR1 antibody (1:100, Proteintech, Rosemont, IL, USA, 23355-1-AP) and flag antibody (1:1000, ThermoFisher Scientific, MA1-91878) overnight at 4 °C. .. After incubation, cells were rinsed with Phosphate-buffered saline (PBS) and incubated with secondary antibodies (1:1000, Cell Signaling Technology, Danvers, MA, USA, 8889S; 4408S) for 1 h, avoiding light exposure.

    Transfection:

    Article Title: KDM6B Variants May Contribute to the Pathophysiology of Human Cerebral Folate Deficiency.
    Article Snippet: .. 48 h after transfection, cells were fixed with 4% paraformaldehyde for 30 min and cell membranns were permeabilized with 0.3% Triton X-100 in TBST for 20 min, and then blocked with 10% normal goat serum (NGS) in Tris-buffered saline with 0.1% Tween® 20 Detergent (TBST) for 1 h. Cells were then incubated with FOLR1 antibody (1:100, Proteintech, Rosemont, IL, USA, 23355-1-AP) and flag antibody (1:1000, ThermoFisher Scientific, MA1-91878) overnight at 4 ◦C. .. After incubation, cells were rinsed with Phosphate-buffered saline (PBS) and incubated with secondary antibodies (1:1000, Cell Signaling Technology, Danvers, MA, USA, 8889S; 4408S) for 1 h, avoiding light exposure.

    Article Title: KDM6B Variants May Contribute to the Pathophysiology of Human Cerebral Folate Deficiency
    Article Snippet: .. 48 h after transfection, cells were fixed with 4% paraformaldehyde for 30 min and cell membranns were permeabilized with 0.3% Triton X-100 in TBST for 20 min, and then blocked with 10% normal goat serum (NGS) in Tris-buffered saline with 0.1% Tween ® 20 Detergent (TBST) for 1 h. Cells were then incubated with FOLR1 antibody (1:100, Proteintech, Rosemont, IL, USA, 23355-1-AP) and flag antibody (1:1000, ThermoFisher Scientific, MA1-91878) overnight at 4 °C. .. After incubation, cells were rinsed with Phosphate-buffered saline (PBS) and incubated with secondary antibodies (1:1000, Cell Signaling Technology, Danvers, MA, USA, 8889S; 4408S) for 1 h, avoiding light exposure.

    Next-Generation Sequencing:

    Article Title: KDM6B Variants May Contribute to the Pathophysiology of Human Cerebral Folate Deficiency.
    Article Snippet: .. 48 h after transfection, cells were fixed with 4% paraformaldehyde for 30 min and cell membranns were permeabilized with 0.3% Triton X-100 in TBST for 20 min, and then blocked with 10% normal goat serum (NGS) in Tris-buffered saline with 0.1% Tween® 20 Detergent (TBST) for 1 h. Cells were then incubated with FOLR1 antibody (1:100, Proteintech, Rosemont, IL, USA, 23355-1-AP) and flag antibody (1:1000, ThermoFisher Scientific, MA1-91878) overnight at 4 ◦C. .. After incubation, cells were rinsed with Phosphate-buffered saline (PBS) and incubated with secondary antibodies (1:1000, Cell Signaling Technology, Danvers, MA, USA, 8889S; 4408S) for 1 h, avoiding light exposure.

    Article Title: KDM6B Variants May Contribute to the Pathophysiology of Human Cerebral Folate Deficiency
    Article Snippet: .. 48 h after transfection, cells were fixed with 4% paraformaldehyde for 30 min and cell membranns were permeabilized with 0.3% Triton X-100 in TBST for 20 min, and then blocked with 10% normal goat serum (NGS) in Tris-buffered saline with 0.1% Tween ® 20 Detergent (TBST) for 1 h. Cells were then incubated with FOLR1 antibody (1:100, Proteintech, Rosemont, IL, USA, 23355-1-AP) and flag antibody (1:1000, ThermoFisher Scientific, MA1-91878) overnight at 4 °C. .. After incubation, cells were rinsed with Phosphate-buffered saline (PBS) and incubated with secondary antibodies (1:1000, Cell Signaling Technology, Danvers, MA, USA, 8889S; 4408S) for 1 h, avoiding light exposure.

    Incubation:

    Article Title: KDM6B Variants May Contribute to the Pathophysiology of Human Cerebral Folate Deficiency.
    Article Snippet: .. 48 h after transfection, cells were fixed with 4% paraformaldehyde for 30 min and cell membranns were permeabilized with 0.3% Triton X-100 in TBST for 20 min, and then blocked with 10% normal goat serum (NGS) in Tris-buffered saline with 0.1% Tween® 20 Detergent (TBST) for 1 h. Cells were then incubated with FOLR1 antibody (1:100, Proteintech, Rosemont, IL, USA, 23355-1-AP) and flag antibody (1:1000, ThermoFisher Scientific, MA1-91878) overnight at 4 ◦C. .. After incubation, cells were rinsed with Phosphate-buffered saline (PBS) and incubated with secondary antibodies (1:1000, Cell Signaling Technology, Danvers, MA, USA, 8889S; 4408S) for 1 h, avoiding light exposure.

    Article Title: KDM6B Variants May Contribute to the Pathophysiology of Human Cerebral Folate Deficiency
    Article Snippet: .. 48 h after transfection, cells were fixed with 4% paraformaldehyde for 30 min and cell membranns were permeabilized with 0.3% Triton X-100 in TBST for 20 min, and then blocked with 10% normal goat serum (NGS) in Tris-buffered saline with 0.1% Tween ® 20 Detergent (TBST) for 1 h. Cells were then incubated with FOLR1 antibody (1:100, Proteintech, Rosemont, IL, USA, 23355-1-AP) and flag antibody (1:1000, ThermoFisher Scientific, MA1-91878) overnight at 4 °C. .. After incubation, cells were rinsed with Phosphate-buffered saline (PBS) and incubated with secondary antibodies (1:1000, Cell Signaling Technology, Danvers, MA, USA, 8889S; 4408S) for 1 h, avoiding light exposure.

    Blocking Assay:

    Article Title: CIC missense variants contribute to susceptibility for spina bifida
    Article Snippet: .. FOLR1 antibody (1:100, Proteintech, 23355-1-AP) was diluted in blocking buffer and incubate overnight at 4. .. 14-16 hours later, cells were rinsed with PBS and incubated with secondary antibody (1:1000, Cell Signaling Technology, 8889S) diluted in blocking buffer for P os te d on A u th or ea 30 N ov 20 21 — T h e co p y ri gh t h ol d er is th e au th or /f u n d er .



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    A. Total spectrum counts of differentially expressed surface proteins between control and radioresistant LNCaP cells is shown. B. FOLR1 expression counts (Log(Fragments per Kilobase million) were plotted using RNA-seq data from prostate tumors available at cBioportal. C . FOLR1 mRNA levels were quantified in LNCaP and LNCaP-RR cells using qPCR. D . FOLR1 mRNA levels were quantified in PC3 and PC3-RR cells using qPCR. E . Surface expression of FOLR1 was quantified in LNCaP, LNCaP-RR, PC3 and PC3-RR cells using flow cytometry.

    Journal: bioRxiv

    Article Title: Folate Receptor α Contributes to Radiation Resistance in Neuroendocrine Prostate Cancer by Regulating Redox Homeostasis

    doi: 10.64898/2026.03.26.714502

    Figure Lengend Snippet: A. Total spectrum counts of differentially expressed surface proteins between control and radioresistant LNCaP cells is shown. B. FOLR1 expression counts (Log(Fragments per Kilobase million) were plotted using RNA-seq data from prostate tumors available at cBioportal. C . FOLR1 mRNA levels were quantified in LNCaP and LNCaP-RR cells using qPCR. D . FOLR1 mRNA levels were quantified in PC3 and PC3-RR cells using qPCR. E . Surface expression of FOLR1 was quantified in LNCaP, LNCaP-RR, PC3 and PC3-RR cells using flow cytometry.

    Article Snippet: We used the following antibodies: Synaptophysin (Thermofisher, SP11, MA5-16402), GAPDH (Cell Signaling, 14C10, 2118S), HIF1alpha (Cell Signaling, 3716S), Vinculin (Abcam, Ab91459), FOLR1-APC (Miltenyi, 130-129-530) and Tubulin (TU-02, SC-8035).

    Techniques: Control, Expressing, RNA Sequencing, Flow Cytometry

    A. HIF-1α protein was quantified in LNCaP and LNCaP-RR cells using immunoblotting. B . HIF-1α mRNA was quantified in LNCaP and LNCaP-RR cells using qPCR. C. HIF-1α protein was quantified in PC3 and PC3-RR cells using immunoblotting. D . HIF-1α mRNA was quantified in PC3 and PC3-RR cells by qPCR. E. Synaptophysin protein expression was quantified by immunoblotting in LNCaP-RR cells after shRNA mediated downregulation of HIF-1α. F. HIF-1α and FOLR1 mRNA expression was quantified by qPCR in LNCaP-RR cells after shRNA mediated downregulation of HIF-1α. G. FOLR1 surface expression was quantified by flow cytometry in LNCaP-RR cells after shRNA mediated downregulation of HIF-1α. H. HIF-1α and FOLR1 mRNA expression was quantified by qPCR in PC3-RR cells after shRNA mediated downregulation of HIF-1α. I. FOLR1 mRNA expression was quantified by qPCR in LNCaP and PC3 cells after culturing cells in CoCl 2 for 2, 4 and 6 days. J. Synaptophysin protein expression was quantified by immunoblotting in LNCaP-RR cells after culturing cells in 1 uM methotrexate for 24h. K. Synaptophysin mRNA expression was quantified by qPCR in LNCaP-RR cells after shRNA mediated downregulation of FOLR1. L. Synaptophysin protein expression was quantified by immunoblotting in PC3 cells after culturing cells in CoCl2 and methotrexate for 24 hours.

    Journal: bioRxiv

    Article Title: Folate Receptor α Contributes to Radiation Resistance in Neuroendocrine Prostate Cancer by Regulating Redox Homeostasis

    doi: 10.64898/2026.03.26.714502

    Figure Lengend Snippet: A. HIF-1α protein was quantified in LNCaP and LNCaP-RR cells using immunoblotting. B . HIF-1α mRNA was quantified in LNCaP and LNCaP-RR cells using qPCR. C. HIF-1α protein was quantified in PC3 and PC3-RR cells using immunoblotting. D . HIF-1α mRNA was quantified in PC3 and PC3-RR cells by qPCR. E. Synaptophysin protein expression was quantified by immunoblotting in LNCaP-RR cells after shRNA mediated downregulation of HIF-1α. F. HIF-1α and FOLR1 mRNA expression was quantified by qPCR in LNCaP-RR cells after shRNA mediated downregulation of HIF-1α. G. FOLR1 surface expression was quantified by flow cytometry in LNCaP-RR cells after shRNA mediated downregulation of HIF-1α. H. HIF-1α and FOLR1 mRNA expression was quantified by qPCR in PC3-RR cells after shRNA mediated downregulation of HIF-1α. I. FOLR1 mRNA expression was quantified by qPCR in LNCaP and PC3 cells after culturing cells in CoCl 2 for 2, 4 and 6 days. J. Synaptophysin protein expression was quantified by immunoblotting in LNCaP-RR cells after culturing cells in 1 uM methotrexate for 24h. K. Synaptophysin mRNA expression was quantified by qPCR in LNCaP-RR cells after shRNA mediated downregulation of FOLR1. L. Synaptophysin protein expression was quantified by immunoblotting in PC3 cells after culturing cells in CoCl2 and methotrexate for 24 hours.

    Article Snippet: We used the following antibodies: Synaptophysin (Thermofisher, SP11, MA5-16402), GAPDH (Cell Signaling, 14C10, 2118S), HIF1alpha (Cell Signaling, 3716S), Vinculin (Abcam, Ab91459), FOLR1-APC (Miltenyi, 130-129-530) and Tubulin (TU-02, SC-8035).

    Techniques: Western Blot, Expressing, shRNA, Flow Cytometry

    A. FOLR1 surface expression was quantified by flow cytometry in LNCaP-RR cells after shRNA mediated downregulation of HIF1A (shRNA-2). B. HIF1A mRNA expression was quantified by qPCR in LNCaP-RR cells after shRNA (shRNA-2) mediated downregulation of FOLR1. C. Synaptophysin protein expression was quantified by immunoblotting in PC3-RR cells after shRNA mediated downregulation of HIF-1α. D. Syp mRNA expression was quantified by qPCR in LNCaP-RR cells after culturing them in CoCl 2 for 24 hours. E. HIF-1α protein levels were quantified by immunoblotting in PC3 cells after culturing cells in CoCl 2 for 2, 4 and 6 days. F. SLC7A11 and GPX4 mRNA expression was quantified by qPCR in LNCaP-RR cells after shRNA mediated downregulation of FOLR1.

    Journal: bioRxiv

    Article Title: Folate Receptor α Contributes to Radiation Resistance in Neuroendocrine Prostate Cancer by Regulating Redox Homeostasis

    doi: 10.64898/2026.03.26.714502

    Figure Lengend Snippet: A. FOLR1 surface expression was quantified by flow cytometry in LNCaP-RR cells after shRNA mediated downregulation of HIF1A (shRNA-2). B. HIF1A mRNA expression was quantified by qPCR in LNCaP-RR cells after shRNA (shRNA-2) mediated downregulation of FOLR1. C. Synaptophysin protein expression was quantified by immunoblotting in PC3-RR cells after shRNA mediated downregulation of HIF-1α. D. Syp mRNA expression was quantified by qPCR in LNCaP-RR cells after culturing them in CoCl 2 for 24 hours. E. HIF-1α protein levels were quantified by immunoblotting in PC3 cells after culturing cells in CoCl 2 for 2, 4 and 6 days. F. SLC7A11 and GPX4 mRNA expression was quantified by qPCR in LNCaP-RR cells after shRNA mediated downregulation of FOLR1.

    Article Snippet: We used the following antibodies: Synaptophysin (Thermofisher, SP11, MA5-16402), GAPDH (Cell Signaling, 14C10, 2118S), HIF1alpha (Cell Signaling, 3716S), Vinculin (Abcam, Ab91459), FOLR1-APC (Miltenyi, 130-129-530) and Tubulin (TU-02, SC-8035).

    Techniques: Expressing, Flow Cytometry, shRNA, Western Blot

    A. FOLR1 mRNA expression was quantified by qPCR in LNCaP-RR cells after shRNA mediated downregulation of FOLR1. B. Clonogenic assay of LNCaP-RR control and shFOLR1 cells after irradiation (0-8 Gy) is shown. C. Clonogenic assay of LNCaP-RR cells treated with 1uM methotrexate after irradiation (0-8 Gy) is shown. D . Overexpression of FOLR1 in PC3 cells was confirmed by flow cytometry. E. Clonogenic assay of PC3 cells (control or overexpressing FOLR1) after irradiation (0-10 Gy) is shown. F. Clonogenic assay of LNCaP-RR cells performed under control or folate-reduced conditions after irradiation (0-8 Gy) is shown. G. Clonogenic assay of PC3-RR cells performed under control or folate-reduced conditions after irradiation (0-10 Gy) is shown.

    Journal: bioRxiv

    Article Title: Folate Receptor α Contributes to Radiation Resistance in Neuroendocrine Prostate Cancer by Regulating Redox Homeostasis

    doi: 10.64898/2026.03.26.714502

    Figure Lengend Snippet: A. FOLR1 mRNA expression was quantified by qPCR in LNCaP-RR cells after shRNA mediated downregulation of FOLR1. B. Clonogenic assay of LNCaP-RR control and shFOLR1 cells after irradiation (0-8 Gy) is shown. C. Clonogenic assay of LNCaP-RR cells treated with 1uM methotrexate after irradiation (0-8 Gy) is shown. D . Overexpression of FOLR1 in PC3 cells was confirmed by flow cytometry. E. Clonogenic assay of PC3 cells (control or overexpressing FOLR1) after irradiation (0-10 Gy) is shown. F. Clonogenic assay of LNCaP-RR cells performed under control or folate-reduced conditions after irradiation (0-8 Gy) is shown. G. Clonogenic assay of PC3-RR cells performed under control or folate-reduced conditions after irradiation (0-10 Gy) is shown.

    Article Snippet: We used the following antibodies: Synaptophysin (Thermofisher, SP11, MA5-16402), GAPDH (Cell Signaling, 14C10, 2118S), HIF1alpha (Cell Signaling, 3716S), Vinculin (Abcam, Ab91459), FOLR1-APC (Miltenyi, 130-129-530) and Tubulin (TU-02, SC-8035).

    Techniques: Expressing, shRNA, Clonogenic Assay, Control, Irradiation, Over Expression, Flow Cytometry

    A. The levels of GSH were quantified in LNCaP and LNCaP-RR cells. B. The relative levels of GSH were quantified in LNCaP-RR cells after shRNA mediated downregulation of FOLR1. C. Relative levels of total ROS measured by DCF fluorescence is compared between LNCaP, LNCaP-RR and LNCaP-shFOLR1 at 24 hrs post radiation (4 Gy). D. Relative levels of total ROS measured by DCF fluorescence in LNCaP-RR cells cultured with or without Methotrexate (1uM) is shown 24 hrs after radiation (4 Gy). E. Bar graph plotted using mean fluorescence intensty to show retention of cell tracer violet dye in control, RR and RR with FOLR1 shRNAs populations of LNCaP cells. F . Level of Ki-67 mRNA levels was quantified by qPCR in control and PC3-RR cells. G . Effect of FOLR1 overexpression in PC3 cells on mRNA levels of Myc, Ki-67 and CDC25 was quantified by qPCR. H. Level of Ki-67 mRNA levels was quantified by qPCR in control, LNCaP-RR and LNCaP-RR-FOLR1shRNAs cells

    Journal: bioRxiv

    Article Title: Folate Receptor α Contributes to Radiation Resistance in Neuroendocrine Prostate Cancer by Regulating Redox Homeostasis

    doi: 10.64898/2026.03.26.714502

    Figure Lengend Snippet: A. The levels of GSH were quantified in LNCaP and LNCaP-RR cells. B. The relative levels of GSH were quantified in LNCaP-RR cells after shRNA mediated downregulation of FOLR1. C. Relative levels of total ROS measured by DCF fluorescence is compared between LNCaP, LNCaP-RR and LNCaP-shFOLR1 at 24 hrs post radiation (4 Gy). D. Relative levels of total ROS measured by DCF fluorescence in LNCaP-RR cells cultured with or without Methotrexate (1uM) is shown 24 hrs after radiation (4 Gy). E. Bar graph plotted using mean fluorescence intensty to show retention of cell tracer violet dye in control, RR and RR with FOLR1 shRNAs populations of LNCaP cells. F . Level of Ki-67 mRNA levels was quantified by qPCR in control and PC3-RR cells. G . Effect of FOLR1 overexpression in PC3 cells on mRNA levels of Myc, Ki-67 and CDC25 was quantified by qPCR. H. Level of Ki-67 mRNA levels was quantified by qPCR in control, LNCaP-RR and LNCaP-RR-FOLR1shRNAs cells

    Article Snippet: We used the following antibodies: Synaptophysin (Thermofisher, SP11, MA5-16402), GAPDH (Cell Signaling, 14C10, 2118S), HIF1alpha (Cell Signaling, 3716S), Vinculin (Abcam, Ab91459), FOLR1-APC (Miltenyi, 130-129-530) and Tubulin (TU-02, SC-8035).

    Techniques: shRNA, Fluorescence, Cell Culture, Control, Over Expression